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Colorimetric measuring principle
 
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Nutrients, metals or carbon components in a liquid can be measured using the colorimetric measuring principle with or without digestion. This video shows what it is about and how this measuring principle work. www.endress.com/analysis
Views: 63652 Endress+Hauser
Colorimetry
 
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Visual examples illustrate Beer's law, namely that absorbance is proportional to both concentration and path length. Our eyes are good colorimeters! This video is part of the Flinn Scientific Best Practices for Teaching Chemistry Video Series, a collection of over 125 hours of free professional development training for chemistry teachers - http://elearning.flinnsci.com ATTENTION: This demonstration is intended for and should only be performed by certified science instructors in a safe laboratory/classroom setting. Be sure to subscribe and check out more videos! Subscribe: https://www.youtube.com/channel/FlinnScientific/ Facebook: https://www.facebook.com/FlinnScientific/ Website: https://www.flinnsci.com/
Views: 49955 FlinnScientific
pH of Buffer Solution by Colorimeteric Method
 
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pH of Buffer Solution by Colorimeteric Method
Views: 336 Edurite
Determination of pH
 
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When an acid is added to pure water, the concentration of H+ ions increases. The ionic product of pure water is 10–14 mol/L at 25°C.
Views: 3270 Tanmay Dixit
colorimeter,how to perform test with colorimeter,practical
 
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Colorimeter used in laboratory मेरा नाम Dinesh kumar है। Channel को subscribe जरूर करें। Video को like share और comments करें। Bmlt,dmlt, mlt, course और compitition exam के लिए books नीचे दिए गए लिंक पर जाकर ले सकते हैं। धन्यवाद। 1.FOR OCHIE BOOK https://amzn.to/2MppW4L 2 FOR GODKER https://amzn.to/2yMJRsl 3 FOR N MURUGESH ANATOMY https://amzn.to/2MqQSBi 4 FOR BIOCHEMISTRY https://amzn.to/2MrDL2A 5 FOR MICROBIOLOGY https://amzn.to/2Kr4IWM 6 FOR HINDI MLT BOOK https://amzn.to/2tKQk1a 7 PHYSIOLOGY https://amzn.to/2KcurTE 8 PATHOLOGY AND HISTOPATHOLOGY https://amzn.to/2MwjXv9 facebook link https://www.facebook.com/profile.php?id=100003527359192 Facebook page- lab technician adda Instagram- lab_ technician_ adda Email- [email protected] is vedio me hamne aapko colorimeter se kaise test perform karte h wo practical karke bataya h pls subscribe kare mere channel ko lab technician adda like my vedio contact me on facebook https://www.facebook.com/profile.php?id=100003527359192
Views: 29099 LAB TECHNICIAN DINESH
Measurement of pH Value
 
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Views: 89781 NCTEL
Colorimetry
 
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A look at how colorimetry is used to continuously monitor the rate of a chemical reaction
Views: 5590 MaChemGuy
How to analyze free chlorine residual
 
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We welcome your feedback and comments! [email protected] To find more resources related to drinking water, please visit our library: www.DrinkingWaterResourceLibrary.ca This video demonstrates how to properly analyze the free chlorine residual of a drinking water sample using the DPD method. This method was adapted from Standard Methods for the Examination of Water and Wastewater. This is a basic procedure overview and should not replace the procedures provided by your equipment manufacturer.
Measurement of Chlorine Residual
 
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This video will cover taking a good chlorine sample and methods for analysis. Effective measurement of chlorine residual is essential for protection of public health. The presence of the residual not only provides disinfection, it also serves and an indicator of water quality. Loss of residual can be an indicator of a water quality problem. Chlorine residual may be measured for compliance or non-compliance purposes. While the analysis will remain the same, how you collect the sample may differ. This video will discuss measurement of chlorine residual using a colorimetry and a handheld spectrophotometer. For more information about chlorine residual and other topics, visit our website at rcap.org.
Membrane Permeability Beetroot Practical
 
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Required practical AS and A level. Investigating the effects of a named variable (temperature) on the permeability of cell surface membranes. Make sure you are familiar with the graph at the end of the clip!
TRU Chemistry Labs: Experiment - Determination of an Indicator Equilibrium Constant - Methyl Red
 
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This video reviews the pre-laboratory material for TRU Chemistry Experiment - Determination of an Indicator Equilibrium Constant - Methyl Red
Views: 4732 TRUChemOnline
Colorimeter
 
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An introduction on how the colorimeter (a Spectronic 20) works and instructions on using it.
Views: 122347 David Frank
Testing Water for Nitrate
 
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Using a Hach DR3900 to test for Nitrate in water
Views: 54180 wildlandschoolvideo
Colorimetry
 
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In this video we discuss colorimetry, which is a technique for quantifying the concentration of a sample of DNA or protein.
Views: 20612 Asterlesma
The Spectrophotometer:  A demo and practice experiment
 
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The spectrophotometer is an instrument used to measure the effect of a sample on a beam of light. We can learn a lot about a substance by studying the way it interacts with light. In this video, we'll demonstrate how to use a spectrophotometer to perform a basic experiment: determining the absorbance spectrum of red food coloring. Included in the video is a short animation that illustrates the inner workings of a spectrophotometer. Special thanks to Joe Issa and the Gaston College Biotechnology department for helping to make this video. Practice using a virtual spec: http://www.ncbionetwork.org/educational-resources/elearning
Views: 306742 BioNetwork
SPECTROPHOTOMETRIC DETERMINATION OF AMMONIA IN WATER
 
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A water sample is treated with a Nessler solution having an exact concentration. The brown or yellowish color suggests the presence of the ammonia in the sample. This color, formed after treating the sample with Nessler's Reagent, is analyzed with a spectrophotometer the result being given in ppm.
Views: 4383 CEEA Erasmus
how to perform colorimetry
 
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Views: 22479 Alec Barron
Make your OWN pH Indicator from Red Cabbage!
 
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Hello everyone. Today we will conduct an experiment that anyone can repeat in their home. We will make a universal pH indicator from a red cabbage. First, we would need to buy the red cabbage. Then we need to cut it into about four parts, and for the experiment one quarter should be enough. Afterwards, further chop the quarter piece into small pieces. The smaller the pieces the better. You can also crush it using a blender. To make the actual indicator, we need to extract purple pigment from the leaves. To do so, put the sliced cabbage in any container and fill it with boiling water. Then wait for about 20 minutes until most of the water-soluble substances appear in the solution. Now I will explain why do we do it. Red cabbage, as well as many other vegetables, contains natural dyes, i.e. anthocyanins, which gives the fruits and leaves their different colors. The color of these substances will depend on the acidity of their environment. When the acidity changes then the color changes as well. For example, the color of ripe fruit often changes from green to red and from yellow to red. Once the cabbage is settled in the water for approximately 20 minutes, the resulting liquid then should be filtered. And, here we’ve made our universal indicator. In order to show its effect, I’ll dilute it in water several times for the solution to not be so saturated. Now I’ll pour this indicator into seven different beakers to show you what colors the substance contained in a red cabbage can acquire. I’ll pour sulfuric acid, a very strong acid, into the first beaker and the solution immediately becomes red while the acidity or pH is equal to one. I’ll add acetic acid to the second beaker, it is not as strong as sulfuric acid and the solution becomes bright pink. And I’ll add sparkling water to the third beaker. Soda contains carbonic acid that turns the solution in a light pink color, with pH being approximately four. I do nothing with the next beaker as there is more or less neutral solution in it. Then, in the following beaker, I’ll add the baking soda solution, the solution turns blue, and the pH is approximately eight or nine now depending on the concentration. In the next beaker I’ll add the ammonia solution, the solution there turns green and the pH is now equal to about twelve or thirteen. Finally, in the last cup I’ll add a strong base - sodium hydroxide, the solution turns green at first and then yellow, yellow indicates pH of about fourteen. Well, and we’ve got a diverse palette from one of the colors. But that's not all, it’s still possible to play around for a long time with this indicator. You can, for example, make an alkaline solution of yellow color and add an acid to it, such as sulfuric acid, and then you can gradually watch all the transitional colors. Or, conversely, it can be an acidic solution, and then adding a solution of an alkali or soda to it. You can also do a multi-layer liquid by pouring the solutions very carefully. I would also want to warn you to be careful when you play about with a variety of substances as, for example, sodium hydroxide and sulfuric acid, are very corrosive substances, if they get on your skin it will be quite painful, so be careful with it. Anthocyanins are the substances that change color and are contained in virtually any brightly colored fruit plants. As well as an indicator you can also use beetroot, redcurrant or carrot juices, thus the diversity is quite large. Facebook: https://www.facebook.com/thoisoi2 Patreon: https://www.patreon.com/Thoisoi?ty=h Music: http://audiomicro.com
Determination of hardness of Water
 
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Views: 412920 NCTEL
Colorimetric measurements
 
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This channel is dedicated to students of chemistry, medicine, pharmacy, biology, physics, agriculture and other branches studying chemistry.
Views: 86 Chemistry Channel
Analyzing Water Samples for Total Phosphorus
 
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Analyzing water samples for total phosphorus at the Wisconsin State Laboratory of Hygiene. Total P_4_Hurley Revisions.mov
Views: 8238 Wisconsin Sea Grant
SPECTROPHOTOMETRIC DETERMINATION OF NITRITES IN WATER
 
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A water sample is treated with a solution capable to do a coupling reaction due to the nitrites content. The pink or red color resulted after the diazotization reaction suggests the presence of the nitrites in the sample. This color is analyzed with a spectrophotometer, the results being given in ppm.
Views: 4992 CEEA Erasmus
Spectrophotometric measurement (Colorimetric method)
 
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Views: 420 Jame
What are Photoelectric colorimeters? Describe the single beam photoelectric colorimeter.
 
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Photoelectric colorimeter is the instruments, with the help of which quantitative analysis of colored solutions is possible. The photoelectric colorimeters are of two types. Single beam photoelectric colorimeters and Double beam photoelectric colorimeters. Single beam photoelectric colorimeter is consisting of the following components:- 1. A source of visible light: An incandescent lamp (on heating glow) with a tungston filament 2. Collimating convex lens: It collects the radiation from source and the transmitted radiation will be traveling parallel to the principle axis because the source is kept exactly at the focus of the lens. 3. Diaphragm: The function of diaphragm is to set 100% transmittance. 4. Filter: It is in the circular disc which is made up of no of colored glass patches. When the light from the source is allow to pass through this filter. The unnecessary radiations are absorbed and we get light of only one colour. 5. Sample Holder OR Cuvette: - It is a rectangular transparent container made up of glass or quartz 6. Photo cell: It is the component which converts the radiations into current. It converts the transmitted beam emerging from sample into an electric current. This electrical pulse is due to the emission of electrons from the photocathode surface caused by the transmitted beam falling on the photo electrode. 7. Signal indicator Read-out meter or digital display: It is a meter calibrated in 0-100 transmittance or absorbance units. Some meter covers both absorbance and transmittance. The optical density or percentage transmittance can be read directly. Light from source such as incandescent lamp with tungsten filament passes through collimating convex lens and then through an adjustable diaphragm. By adjusting this diaphragm, the intensity of incident radiation can be altered to any required level. Light is then incident on a filter which allows only a narrow band of wave lengths to pass through it in the curette. The solvent or the sample solution is placed in the curette and transmitted light falls on the photo Cell producing small current. The current is then incident on Read out meter or dial, which reads transmittance or OD. First we take blank i.e. solvent in the cuvette which has been properly cleaned and insert it in the optical path of the instrument. As it is blank therefore no absorption and is fully is transmitted and then incident on photocell and then dial. Adjust the reading to O.D. zero or 100% transmittance. Now remove the blank from cuvette and put the solution. When the light is incident on it some radiation are absorbed and the remaining is transmitted which is incident on photocell and then on Dial which Will give O.D. or transmittance. If O.D. or transmittance is known then by using Beer-Lambert's law. Single beam photo electric colorimeters have following disadvantages:- As the source of radiations is AC. i.e. alternating Current i.e. Fluctuations in the voltage causes fluctuations in reading. ii) As the solvent contain the impurities, which also absorb the radiations. Therefore, it is not possible to get the correct amount of light absorbed. iii) As filter is used which do not give 100% monochromatic light. Therefore analysis is not accurate has error. Chemical Thermodynamics - Physical Chemistry https://www.youtube.com/watch?v=cATdLP86uHQ&list=PL9AUXQTZw3SsLGGp8p6xeGwM3P9adIRR1 Ionic Equilibria - Physical Chemistry https://www.youtube.com/watch?v=llTs2-BMmgA&list=PL9AUXQTZw3SuWLAc0-MNpD8Vc-7LyN52w Electrochemistry - Physical Chemistry https://www.youtube.com/watch?v=ifdy9wnF80s&list=PL9AUXQTZw3Sssn-kip7xzKGzwljSRPEX2 Solid State - Physical Chemistry https://www.youtube.com/watch?v=Pz-yaCYkHZI&list=PL9AUXQTZw3SuoPwu8UFZq4zXiPEC-So6i Gaseous State - Physical Chemistry https://www.youtube.com/watch?v=bvp0d8qZCpE&list=PL9AUXQTZw3Ssp6Ne6_XbBu8TLuO7qEd4L Colloidal States - Physical Chemistry https://www.youtube.com/watch?v=yjeFUF-fNLQ&list=PL9AUXQTZw3SszL0qFEw1jciS3QIXZB4aZ Stereochemistry - Organic Chemistry https://www.youtube.com/watch?v=N5GU6379LCo&list=PL9AUXQTZw3Su_8A-izFKGfBJvSZY7XcCx Nanomaterials - Engineering Chemistry https://www.youtube.com/watch?v=eDVwEf2PgRI&list=PL9AUXQTZw3SsYzEUlxhIzmVCvRcyCy9fh Water and Its Treatment - Engineering Chemistry https://www.youtube.com/watch?v=eOsZ12nuJnA&list=PL9AUXQTZw3Ss8p3l-b_YIkD-8ZvX4YDsy Electrochemistry - Engineering Chemistry https://www.youtube.com/watch?v=oleTF2Qw-gg&list=PL9AUXQTZw3SuETg2vcx6uEYKFwBVJtEot Environmental Studies https://www.youtube.com/watch?v=-jm3JOcUwdQ&list=PL9AUXQTZw3SvK27T5RCYJoP_NpndyuEs2 Optics - Applied Physics https://www.youtube.com/watch?v=XUw7C4AE0Hk&list=PL9AUXQTZw3StU-aX5h47EZTEkOteCjdz6 For Details Visit http://cepekmedia.co.nf http://cepek.hol.es/ http://edmerls.66Ghz.com/ http://edmerls.tk/ #Colorimeter #AnalyticalChemistry #Chemistry #BeerLambertsLaw #Spectroscopy
Views: 2722 Cepek Media
Quantitative Estimation of Aminoacids by Ninhydrin Method - Amrita University
 
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▶ This video channel is developed by Amrita University's CREATE http://www.amrita.edu/create ▶ Subscribe @ https://www.youtube.com/user/amritacreate http://www.youtube.com/amritavlab ▶ Like us @ https://www.facebook.com/CREATEatAmrita ▶ For more Information @ http://vlab.amrita.edu/index.php?sub=3&brch=63&sim=156&cnt=1 ▶ Amrita Virtual Lab Project website http://vlab.amrita.edu Amino acids are known to be the building block of all proteins. Twenty different amino acids are commonly found in proteins. Amino acids comprises of a carboxyl group and an amino group bonded to the same carbon atom (the α carbon).They differ from each other in their side chains (R groups),which vary in structure, size, electric charge and solubility in water. Hence their detection, quantification and identification in any sample constitute important steps in the study of proteins. Ninhydrin (triketohydrindene hydrate), a powerful oxidizing agent reacts with all amino acid between Ph 4 and 8 to give purple coloured compound. The reaction is also given by primary amino and ammonia, but without the liberation of CO2. Heating in the presence of Ninhydrin causes oxidative deamination of the alpha amino group amino acids and peptides with the concomitant reduction of Ninhydrin molecule. The reduced Ninhydrin react with ammonia and with a molecule of the oxidized Ninhydrin to yield coloured compound (Ruhemann's violet-blue complex).
Views: 38654 Amrita Vlab
Spectrophotometry introduction | Kinetics | Chemistry | Khan Academy
 
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Spectrophotometry, transmittance, absorbance and the Beer-Lambert Law. Created by Sal Khan. Watch the next lesson: https://www.khanacademy.org/science/chemistry/chem-kinetics/spectrophotometry-tutorial/v/spectrophotometry-example?utm_source=YT&utm_medium=Desc&utm_campaign=chemistry Missed the previous lesson? https://www.khanacademy.org/science/chemistry/chem-kinetics/arrhenius-equation/v/catalysts?utm_source=YT&utm_medium=Desc&utm_campaign=chemistry Chemistry on Khan Academy: Did you know that everything is made out of chemicals? Chemistry is the study of matter: its composition, properties, and reactivity. This material roughly covers a first-year high school or college course, and a good understanding of algebra is helpful. About Khan Academy: Khan Academy offers practice exercises, instructional videos, and a personalized learning dashboard that empower learners to study at their own pace in and outside of the classroom. We tackle math, science, computer programming, history, art history, economics, and more. Our math missions guide learners from kindergarten to calculus using state-of-the-art, adaptive technology that identifies strengths and learning gaps. We've also partnered with institutions like NASA, The Museum of Modern Art, The California Academy of Sciences, and MIT to offer specialized content. For free. For everyone. Forever. #YouCanLearnAnything Subscribe to Khan Academy’s Chemistry channel: https://www.youtube.com/channel/UCyEot66LrwWFEMONvrIBh3A?sub_confirmation=1 Subscribe to Khan Academy: https://www.youtube.com/subscription_center?add_user=khanacademy
Views: 492111 Khan Academy
Iron test by spectrophotometer in Hindi
 
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Iron test using phenonthroline method by spectrophotometer as per BIS/APHA menthd in Hindi
Calcium-EDTA titration
 
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This video demonstrates the titration of calcium with an EDTA titrant. The indicator used is another chelating agent, Eriochrome Black T. The color transition can be very difficult to see due to the very gradual change in color of the indicator over the course of the titration.
Views: 182741 crharrison
Bradford Assay
 
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For more information, visit http://www.bio-rad.com/yt/got-protein. This video demonstrates how to quantitate proteins using the Bio-Rad Got Protein?™ kit. Protein concentration is determined both visually by comparing samples to a set of standards and by measuring absorbance on a SmartSpec™ spectrophotometer. http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=4dad28a9-0b07-49f2-b399-9e5b55643b04&WT.mc_id=yt-lse-ww-biotech-20121012-vfY3mVOlGBU We Are Bio-Rad Explorer. Our Mission: Bio-Rad’s Explorer program provides easy access to engaging hands-on science learning experiences that spark interest in science and its influence on the world. To do this we: Provide high quality, relevant, learning experiences based on real world science Empower educators with skills and confidence to deliver engaging, memorable lessons Connect with Bio-Rad Explorer Online: Website: http://www.bio-rad.com/en-us/education Twitter: https://twitter.com/BioRadEducation Pinterest: https://www.pinterest.com/bioradexplorer/ LinkedIn: https://www.linkedin.com/company/1613226/ Facebook: https://www.facebook.com/biorad/ Instagram: @BioRadLabs Snapchat: @BioRadLabs
Views: 99646 Bio-Rad Laboratories
A simplified method of predicting the colorimetry of spot colour overprints
 
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Kiran Deshpande Phil Green, London College of Communication Zeeba TV (http://zeeba.tv) is part of the River Valley group of Companies. http://www.rivervalleytechnologies.com/
Views: 260 rivervalleytv
Color Measurement for the Food and Beverage Industry
 
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In the food and beverage industry, color and appearance are key ingredients to a product’s success. When the color is off or different from what you expect, your mind might say it tastes different too. From raw material to final product, Konica Minolta Sensing Americas offers instrument and software solutions used to effectively control the color quality and consistency of food and beverages. This high precision technology allows users to evaluate color attributes of various samples, including solids, liquids, powders, pastes, and granules.You will identify any inconsistencies immediately in a more efficient and streamlined process. In addition to meeting color quality standards and expectations, waste is reduced and profits are increased. http://sensing.konicaminolta.us/industries/food-and-beverage/
Chemical Oxygen Demand (COD) Measurement
 
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A chemical oxygen demand test is used to measure the amount of organic compounds in a water sample. It measures the capacity of water to consume oxygen during the decomposition of organic matter and the oxidation of inorganic chemicals such as ammonia and nitrate. COD testing is relatively easy you just need to use good laboratory practice and follow the set stages of the method. Before you start, make sure you choose the COD vials that will measure over the range you expect to find in your water sample. You can see the range of chemical oxygen demand vials we have on our website here: http://www.camlab.co.uk/cod-vials-for-chemical-oxygen-demand-testing-c2866.aspx For more information on COD measurement please get in touch with our support team on 01954 233 120 or email us [email protected] and we can help you out.
Views: 32782 Camlab Ltd
Determination of Alkalinity in the Water
 
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Laboratory equipment: - Erlenmeyer flasks - pipettes - safety bulb - burettes Chemicals: - 0.1 M HCl - indicators: phenolphthalein and methyl orange Procedure: We've put 50ml of water in an Erlenmeyer flask and added 3 drops of phenolphthalein indicator to determine phenolphthalein alkalinity. The color had not changed to purple, which means that we didn't have to titrate with HCl. Then we added 3 drops of methyl orange indicator to determine the total alkalinity, the colour of our solution changed to yellow. The final step was to titrate our solution with 0.1M HCl until the color changes to orange. The result: With this method we found that our sample of water contains hydrogen carbonate and carbonate ions.
Views: 19763 Kristina Kezerić
What is BERTHELOT'S REAGENT? What does BERTHELOT'S REAGENT mean? BERTHELOT'S REAGENT meaning
 
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What is BERTHELOT'S REAGENT? What does BERTHELOT'S REAGENT mean? BERTHELOT'S REAGENT meaning - BERTHELOT'S REAGENT definition - BERTHELOT'S REAGENT explanation. Source: Wikipedia.org article, adapted under https://creativecommons.org/licenses/by-sa/3.0/ license. SUBSCRIBE to our Google Earth flights channel - https://www.youtube.com/channel/UC6UuCPh7GrXznZi0Hz2YQnQ Berthelot's reagent is an alkaline solution of phenol and hypochlorite, used in analytical chemistry. It is named after its inventor, Marcellin Berthelot. Ammonia reacts with Berthelot's reagent to form a blue product which is used in a colorimetric method for determining ammonia. The reagent can also be used for determining urea. In this case the enzyme urease is used to catalyze the hydrolysis of urea into carbon dioxide and ammonia. The ammonia is then determined with Berthelot's reagent. Phenol in the Berthelot reagent can be replaced by a variety of phenolic reagents, the most common being sodium salicylate, which is significantly less toxic. This has been used for blood urea nitrogen (BUN) determinations and commonly is used to determine water and soil total and ammonia-N. Replacement of phenol by 2-phenylphenol reduces interferences by a variety of soil and water constituents and improves color stability at slightly lower pH. Berthelot's reagent has been used in a range of situations. It is often used in colorimetric methods, through an AutoAnalyzer, spectrophotometer, or multiwell plate spectrophotometer. The reagent lacks sensitivity in situations where there may be amines as well as ammonia, however this can be overcome in part by the use of 2-pheylphenol to replace phenol. An ion selective electrode, or distillation/titration method can often be used in cases where Berthelot chemistry is ineffective. Berthelot chemistry has also been adapted for the analysis of nitrite and nitrate in soil and water after conversion, typically by reduction with Devarda's alloy, of these species to ammonium.
Views: 674 The Audiopedia
A.8.6 Find the  concentration of a solution via calibration curve (Beer-Lambert law) IB Chemistry HL
 
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Simple: 1) Find the most absorbed wavelength in your sample using a spectrometer. 2) Accurately measure the colour of multiple concentrations of your sample. 3) Plot a graph of concentration against concentration -- tah dah you have a calibration curve based on the Beer-Lambert Law. Now you can find the unknown concentrations of other samples. Tricky: Absorbance = log Io/ I = elc where Io= intensity of incoming light I=intensity of outgoing light e= constant for the substance l =path length of light through the substance c=concentration of substance It's in the data book!
Views: 118510 Richard Thornley
Colorimetrix for BSA colorimetric test
 
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Colorimetrix in action measuring a commercial Bovine Serum Albumin test (BSA). The app is available for research proposes, should you like to partner with us please visit www.colorimetrix.com for more information. Thanks to Dr Hsin-Yu Fang at TUMunich for providing the test. Music track from creative commons license https://soundcloud.com/vondopejams/
Views: 2949 Colorimetrix
Construction of Protein Standard Curve - Amrita University
 
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▶ This video channel is developed by Amrita University's CREATE http://www.amrita.edu/create ▶ Subscribe @ https://www.youtube.com/user/amritacreate http://www.youtube.com/amritavlab ▶ Like us @ https://www.facebook.com/CREATEatAmrita ▶ For more Information @ http://vlab.amrita.edu/index.php?sub=3&brch=64&sim=1087&cnt=1 ▶ Amrita Virtual Lab Project website http://vlab.amrita.edu A standard curve is a quantitative research tool, a method of plotting assay data that is used to determine the concentration of a substance, particularly proteins and DNA.Lowry's assay for total protein is one of the most commonly performed colorimetric assays. Protein reacts with the Folin- lowry reagent to gives coloured complex. The colour so formed is due to the reaction of the alkaline copper with the protein as in biuret test and the reduction of phosphomolybdate by tyrosine and tryptophan present in the protein. The intensity of colour depends on the amount of these aromatic amino acid present and will vary in different protein
Views: 14550 Amrita Vlab
Haemoglobin Estimation- Sahli's Method (हीमोग्लोबिन टेस्ट)
 
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Want to purchase this, use the link- https://amzn.to/2LG1PyX Haemoglobin is the major component of blood which carries oxygen to the tissue for various function. when that goes down below the normal level one feels lethargy and fatigue. it's always better to estimate the haemoglobin level in blood. After watching this video you will be able to understand- what is haemoglobin test, haemoglobin experiment, haemoglobin in Hindi, Sahli's hemoglobinometer, how to check haemoglobin in blood, haemoglobin estimation test, find your haemoglobin count, haemoglobin level in man, haemoglobin level in the woman, Haemoglobin can be estimated by two methods- Sahli's Haemoglobinometer and hemoglobin colour scale. Step by steps procedure to estimate haemoglobin in blood by Sahli's method. 1- Take out the Haemoglobinometer and arrange its component in stable place. 2.Prepare 0.1N HCl 3.Fill the 0.1 N into Sahli Haemometer tube up to 2% mark 4. Now place the pipette in Sahli Haemometer at a given place (in between two windows) 5. Now sterilize the fingertip and prick it with the help of sterilized lancet and fill the blood into Sahli Haemometer pipette up to 20 microliter marks. 6.Now transfer that blood sample into Sahli Haemometer tube in which we have already taken 0.1 N HCl. 7.Wait for about 10 minutes to let the reaction occur. 8.Now slowly-slowly dropwise add distilled water to its side and shake well with the help of given glass rod. 9.Keep adding distilled water till the color of blood match the side window 10. Observe and note down the reading, that’s the percentage of hemoglobin हेमोग्लोबिन रक्त का प्रमुख घटक है जो विभिन्न कार्यों के लिए ऊतक को ऑक्सीजन लेता है। जब यह सामान्य स्तर से नीचे चला जाता है तो कोई सुस्त और थकान महसूस करता है। रक्त में हीमोग्लोबिन स्तर का अनुमान लगाने के लिए हमेशा बेहतर होता है। इस वीडियो को देखने के बाद आप समझ पाएंगे- हेमोग्लोबिन परीक्षण, हेमोग्लोबिन प्रयोग, हिंदी में हीमोग्लोबिन, सहली के हीमोग्लोबिनोमीटर, रक्त में हीमोग्लोबिन की जांच कैसे करें, हीमोग्लोबिन अनुमान परीक्षण, अपने हीमोग्लोबिन गिनती, हेमोग्लोबिन स्तर में आदमी, हीमोग्लोबिन स्तर औरत, हेमोग्लोबिन का अनुमान दो तरीकों से किया जा सकता है- सहली के हीमोग्लोबिनोमीटर और हीमोग्लोबिन रंग पैमाने। साहली की विधि द्वारा रक्त में हीमोग्लोबिन का अनुमान लगाने के लिए कदम प्रक्रियाओं के चरण। 1- हेमोग्लोबिनोमीटर निकालें और इसके घटक को स्थिर स्थान पर व्यवस्थित करें। 2. 0.1 एन एचसीएल तैयार करें 3. 0.1 एन को सहली हैमोमीटर ट्यूब में 2% तक भरें 4. अब दिए गए स्थान पर सहली हैमोमीटर में पिपेट रखें (दो खिड़कियों के बीच में) 5. अब उंगलियों को निर्जलित करें और नसबंदी वाले लेंस की मदद से इसे छेड़छाड़ करें और रक्त को 20 माइक्रोलिटर अंकों तक सहली हैमोमीटर पिपेट में भरें। 6. अब हस्तांतरण करें कि सहली हैमोमीटर ट्यूब में रक्त नमूना जिसमें हम पहले ही 0.1 एन एचसीएल ले चुके हैं। 7. प्रतिक्रिया होने के लिए लगभग 10 मिनट के लिए प्रतीक्षा करें। 8. धीरे धीरे धीरे-धीरे ड्रॉपवाइड अपनी तरफ आसुत पानी जोड़ें और दी गई ग्लास रॉड की मदद से अच्छी तरह से हिलाएं। 9. खून के रंग से तरफ खिड़की से मेल खाने तक आसुत पानी डालना रखें 10. पढ़ना और पढ़ना नोट करें, यह हीमोग्लोबिन का प्रतिशत है Get in touch with the solution by just clicking following links- Facebook page- https://www.facebook.com/pharmavideo/ Facebook Group- https://www.facebook.com/groups/solutionpharamcy Instagram- https://www.instagram.com/solutionpharmacy/
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Colorimeter 3: Determining the percentage of iron in aluminium foil.
 
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Please visit my websites at http://www.flashscience.co.uk and http://20to9.com/youtube_database.html for more helpful chemistry video resources. Thank you! Finding the perentage of iron in aluminium foil. The foil is dissolved in concentrated hydrochloric acid forming the chlorides of the metals. The iron (II) chloride is oxidised to iron (III) using either hydrogen peroxide or concentrated nitric acid. Potassium thiocyanate is then added to produce the blood-red iron (III) thiocyanate complex ion in solution. The mass of iron in the foil is then determined by colorimetry. Presentation and Production: Frank Scullion
Views: 2567 FranklyChemistry
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Wireless Colorimeter and Turbidity Sensor Overview
 
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PASCO's Wireless Colorimeter and Turbidity Sensor can measure the transmittance and absorbance of colored solutions and determine the turbidity of water samples. Learn more: Wireless Colorimeter and Turbidity Sensor: http://pasco.com/go?PS-3215 SPARKvue software: http://pasco.com/sparkvue
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Project Name: Creation of e-Contents on fermentation technology Project Investigator: Dr. Ramesh Kothari Module Name: Estimation of alcohol
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Determination of copper
 
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Iodometric determination of copper is based on the oxidation of iodides to iodine by copper (II) ions, which get reduced to Cu+. Supplies: Flask with copper solution Erlenmeyer Pipette Burette Reactions: 2Cu2+ + 4I- → 2CuI(s) + I2 2S2O32- + I2 → S4O62- + 2I- Procedure: First we added distilled water in blue solution of copper to get total of 100 mL diluted solution. Using the pipette we took 25,0 mL aliquot of the diluted copper solution and poured it into Erlenmeyer. We added concentrated ammonia to neutralize the acid solution and then added 5 mL of concentrated acetic acid and 3 g of solid potassium iodide which made our solution brown. Now we titrated that brown solution using the thiosulfate solution as a titrant until it turned yellow. We added 3 mL of starch solution and titrated it again until the blue colour was gone. Now we added 2 g of potassium thiocyanate and, since the blue colour was back we titrated it again until the solution was white. Participants: Dorotea Gajdek (advanced group) Marina Gapit (advanced group) Juraj Milačić (advanced group) Ema Pollak (beginners group) Valentino Subotičanec (begginers group)
Views: 12912 Dorotea Gajdek

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