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The Microbiome and the Human Microbiome Project
 
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In this clip (4 of 10), Dr. Hibberd provides a simplified explanation of the human microbiome and discusses analyzing microbial samples for their genetic content, including for comparison with databases. This clip is part of the lecture "Probiotics, the Microbiome, and Host Immune Response: Insights for Novel Therapeutic Approaches," by Patricia Hibberd, M.D., Ph.D., professor of pediatrics and chief of the Division of Global Health, Department of Pediatrics, at Harvard Medical School, as well as professor of global health at Harvard School of Public Health. This 2013 lecture, delivered at NIH, is part of the NCCAM Online Continuing Education Series. Free CME/CEU credit is available to health professionals (see http://nccam.nih.gov/training/videolectures).
Views: 5687 NCCIH
Microbiome/Metagenome Analysis Workshop: QIIME
 
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Analysis of 16S data using QIIME presented by Kellyanne Duncan. For more info: https://www.brown.edu/academics/computational-molecular-biology/cbc-microbiomemetagenome-analysis-workshop Tuesday, November 7th 2017 Brown University
Views: 3594 Brown University
2.11 - What is a patient? Development and the microbiota
 
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"Evolutionary Medicine" Sinauer Associates (2015) is the textbook that supports these lectures. Instructors can request examination copies and sign up to download figures here: http://www.sinauer.com/catalog/medical/evolutionary-medicine.html
Views: 1106 YaleCourses
Functional Analysis of Human Microbiome, Metagenomes, Metatranscriptomics... - Curtis Huttenhower
 
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July 24-26, 2013 - Human Microbiome Science: Vision for the Future More: http://www.genome.gov/27554404
Functional dynamics of the gut microbiome in health and disease
 
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Functional dynamics of the gut microbiome in health and disease Air date: Tuesday, October 27, 2015, 3:00:00 PM Category: WALS - Wednesday Afternoon Lectures Runtime: 01:00:59 Description: NIH Director’s Wednesday Afternoon Lecture Series Dr. Fraser's current research interests are focused oncharacterization of the structure and function of the microbial communitiesthat are found in the human environment, as part of the NIH-funded HumanMicrobiome Project, including projects specifically focused on obesity,metabolic syndrome, inflammatory bowel disease, the interactions between thehuman immune response and the gut microbiome, and the impact of probiotics onthe structure and function of the intestinal microbiome. About the annual Rolla E. Dyer lecture: The annual Rolla E. Dyer Lecture features aninternationally renowned researcher who has contributed substantially to themedical as well as the biological knowledge of infectious diseases. Establishedin 1950, the lecture series honors former NIH director Dr. Dyer, who was anoted authority on infectious diseases. For more information go to https://oir.nih.gov/wals Author: Claire Fraser, Ph.D., Professor of Medicine, Microbiology and Immunology; Director, Institute for Genome Sciences; University of Maryland School of Medicine Permanent link: http://videocast.nih.gov/launch.asp?19272
Views: 2372 nihvcast
16S rRNA Gene Sequencing Service
 
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Introduction The 16S rRNA gene is a highly conserved component of the transcriptional machinery of all DNA-based life forms and thus is highly suited as a target gene for sequencing DNA in samples containing up to thousands of different species. 16S rRNA gene sequencing is commonly used for identification, classification and quantitation of microbes in complex biological mixtures such as environmental samples like water, soil or air, and microbiome samples such as milk or feces. Additionally, with the multiplexing of many samples and high depth of coverage afforded by today’s next-gen platforms, we can now analyze samples from comprehensive time series to quantify microbial community dynamics across many sites, or produce detailed 3D maps of microbial communities, as well as explore whether changes in rare or abundant species are associated with health and disease. Conveniently, the 16S ribosomal RNA gene consists of both highly conserved and variable regions. Universal PCR primers can be designed to target the conserved regions of 16S making it possible to amplify the gene in a wide range of different microorganisms from a single sample. While the conserved region makes universal amplification possible, subsequent sequencing of the variable regions allows discrimination between specific microorganisms such as bacteria, archaea and microbial eukarya. LC Sciences offers a comprehensive 16S ribosomal RNA gene sequencing service for identification and classification of species in microbial samples. LC Sciences has developed a dual zone amplification strategy, targeting the V3 and V4 variable regions. Sample bar-coding enables multiplexing of hundreds of different samples in a single sequencing run, keeping per sample costs low. Sequencing is performed on the industry leading Illumina MiSeq platform and extensive data analysis is provided including: sequencing data output statistics, sequence clustering into operational taxonomic units or OTUs, diversity analysis, classification and abundance analysis. An important distinction of LC Sciences’ service is that we perform the classification analysis on 7 taxonomy levels, all the way down to species level. Species level ID is enabled through the curation of several genomic databases and advanced bioinformatics. OTU Analysis An operational taxonomic unit or OTU is a measure of microbial diversity commonly used when performing 16S sequencing analysis. Sequence data is clustered into OTUs based on sequence similarity and each OTU generally represents a distinct species. OTU Venn diagrams can be used to show the unique OTUs in different subsets of sample groups. Species Accumulation Curve Statistical analysis of OTU data also generates a species accumulation curve which is a method to estimate the number of additional OTUs that may be discovered through further effort and can be an indication of adequacy or deficiency in number of samples analyzed, similar to power analysis. Diversity Analysis LC Sciences’ comprehensive service includes both Alpha and Beta diversity analysis. Alpha diversity is a measurement of the variety of organisms that inhabit a defined region or habitat. LC Sciences calculates diversity based on the Shannon, Simpson, Chao1 and observed species indices. Rarefaction Curve & Rank Abundance Curve The diversity among samples can be observed from the rarefaction curves of each sample and the rank abundance curve indicates species abundance and uniformity. Beta Diversity Analysis Beta diversity represents the explicit comparison of microbial (or other) communities based on their composition. Beta diversity metrics thus assess the differences between microbial communities. Principal Coordinates Analysis Clustering analysis of beta diversity data by the UPGMA algorithm is performed to plot a phylogenetic tree and Principal Coordinates Analysis is performed to visualize and explore similarities or dissimilarities of data. Classification of Results by Taxonomic Level The taxonomic analysis is performed by mapping OTU representative tags to the RDP, Greengenes, and NCBI 16S microbial databases. Taxonomy results are displayed in the form of area charts, heat map, and phylogenetic trees. Note the heatmaps are plotted on 7 taxonomic levels, all the way down to species level. The phylogenetic trees show the phylogenesis among species. The tree is composed of nodes that represent taxonomic units (from phylum through genus), and branches that denote the phylogenetic relation between nodes. LC Sciences comprehensive data report is presented in a user friendly HTML format for easy visualization and interpretation of your experimental results.
Views: 8494 LC Sciences
Approaches to Microbiome Analysis
 
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NGS Leaders is a community created to advance the use and value of next-generation sequencing through knowledge sharing. Join now to network with your peers and help drive the future of genomics.
Views: 1550 cambhealthassoc
Molecular Data & the Microbiome
 
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Curtis Huttenhower is an Associate Professor of Computational Biology and Bioinformatics at the Harvard T.H. Chan School of Public Health and an Associate Member at the Broad Institute. In this video Dr. Curtis Huttenhower will present a lecture titled, “Molecular Data and the Microbiome.” Video Description High-throughput sequencing has become one of many technologies that can be integrated to enable culture-independent studies of microbial communities. These include both environmental microbes, such as those that influence agriculture or water quality, as well as the human microbiome and its roles in health and disease. The multi'omic combination of amplicon sequencing, metagenomics, metatranscriptomics, and other functional molecular data is helping to bridge "parts lists" of these microbial residents with their phenotypic and environmental effects. This raises two current computational challenges: how to provide the most precise bioinformatic solutions describing microbial community systems biology, and what new applications of these integrated data types can be developed to improve population health? Dr. Huttenhower will discuss computational profiling methods for the human microbiome, which provide strain-specific microbial identification and metabolic reconstruction. In combination with microbiome-appropriate statistical tools, these can thus associate microbial features with health outcomes and covariates in human populations to better understand immune and inflammatory disease. About the Speaker Dr. Curtis Huttenhower is an Associate Professor of Computational Biology and Bioinformatics at the Harvard T. H. Chan School of Public Health and an Associate Member at the Broad Institute. He was an analysis lead in the NIH Human Microbiome Project, the "HMP2," and currently leads the Human Microbiome Bioactives Resource. His lab focuses on computational methods for functional analysis of microbial communities, as well as microbiome epidemiology to link microbial community function to public health. View slides from this lecture: https://drive.google.com/open?id=1U36NyMinKAm1R2rwgRb0fJUFVHbeYS9J Visit our webpage to view archived videos covering various topics in data science: https://bigdatau.ini.usc.edu/data-science-seminars
Genomics of Microbes and Microbiomes (2010)
 
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March 16, 2010 Julie Segre, Ph.D Current Topics in Genome Analysis 2010 Handout: http://www.genome.gov/Pages/Research/IntramuralResearch/DIRCalendar/CurrentTopicsinGenomeAnalysis2010/CTGA2010_Lec09_color.pdf More: http://www.genome.gov/12514286
Spatial and Temporal Structure in the GI Microbiota
 
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Authors: Eric J. de Muinck, Knut E. A. Lundin, Pål Trosvik Editor: Morgan G. I. Langille Importance: The human gut microbiome is the subject of intense study due to its importance in health and disease. The majority of these studies have been based on the analysis of feces. However, little is known about how the microbial composition in fecal samples relates to the spatial distribution of microbial taxa along the gastrointestinal tract. By characterizing the microbial content both in intestinal tissue samples and in fecal samples obtained daily, we provide a conceptual framework for how the spatial structure relates to biotic interactions on the community level. We further describe general categories of spatial distribution patterns and identify taxa conforming to these categories. To our knowledge, this is the first study combining spatial and temporal analyses of the human gut microbiome. This type of analysis can be used for identifying candidate probiotics and designing strategies for clinical intervention. DOI: https://doi.org/10.1128/mSystems.00086-17 Published in mSystems® on 5 September 2017 Subscribe to ASM's YouTube channel at https://goo.gl/mOVHlK Learn more about the American Society for Microbiology at http://www.asm.org Become a member today at http://www.asmscience.org/join Interact with us on social at: Facebook Show your support and get updates on the latest microbial offerings and news from the ASM. http://www.facebook.com/asmfan ASM International Facebook Groups Join an ASM International Facebook Group and connect with microbiologists in your region. http://www.asm.org/index.php/programs/asm-international-facebook-groups Twitter Follow all the latest news from the Society. http://www.twitter.com/ASMicrobiology Instagram Outstanding images of your favorite viruses, fungi, bacteria and parasites http://www.instagram.com/asmicrobiology/
Katherine Pollard: "Massive Data Sheds Light on Your Microbiome" | Talks at Google
 
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The most influential “organ” in the human body might be made up of foreign cells—six pounds worth of microorganisms. Katherine Pollard discusses how her lab at the Gladstone Institutes uses big data and high-performance computing to study the human microbiome and learn how it influences health and disease. The human microbiome plays a role in processes as diverse as metabolism, immune function, and mental health. Yet despite the importance of this system, scientists are just beginning to uncover which microorganisms reside in and on our bodies and determine what functions they perform. The development of innovative technology and analytical methods has enabled researchers like Dr. Pollard to decode the complex interactions between our human cells and microbial brethren, and infer meaning from the staggering amounts of data 10 trillion organisms create. Katherine Pollard, PhD, is a senior investigator at the Gladstone Institutes, director of the Gladstone Convergence Zone, and a professor of epidemiology and biostatistics at UCSF.
Views: 6788 Talks at Google
Genomics of Microbes and Microbiomes - Julia Segre (2014)
 
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June 4, 2014 - Current Topics in Genome Analysis 2014 A lecture series covering contemporary areas in genomics and bioinformatics. More: http://www.genome.gov/COURSE2014
Computing Primetime: Decoding the Microbiome
 
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(Visit: http://www.uctv.tv/) UC San Diego Qualcomm Institute’s Larry Smarr, noted authority in information technology and high-performance computing hosts a discussion with UC San Diego’s Rob Knight, leading expert on microbiomes and bioinformatics who is widely renowned for his early and innovative investigations of the symbiotic relationships between microbial life and humans, about how the unique cyberinfrastructure resources for Big Data at UC San Diego will drive applications in the new frontier of microbiome research. Series: "Computing Primetime" [4/2016] [Science] [Show ID: 29711]
Keynote 1: Diversity, Stability and Resilience of the Microbiome - David Relman
 
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July 24-26, 2013 - Human Microbiome Science: Vision for the Future More: http://www.genome.gov/27554404
Microbiome/Metagenome Analysis Workshop: Introduction to Metagenomics
 
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Presented by Christopher Hemme, PhD, Bioinformatics Core Coordinator at URI RI-INBRE For more info: https://www.brown.edu/academics/computational-molecular-biology/cbc-microbiomemetagenome-analysis-workshop Tuesday, November 7th 2017 Brown University
Views: 1445 Brown University
Gabriele Berg. The Power of Networking: Studying Microbiomes for Health
 
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The plant microbiome is a key determinant of plant health and productivity. The microbiomes associated with plants form tight networks, which revealed strong species and niche specialization. Analyzing microbiome networks from healthy and diseased plants led to the identification of health indicators and pathogen-supporting microorganisms as well. This approach thus serves to open new opportunities for future targeted biocontrol studies and could fuel progress in sustainable agriculture, such as the development of microbial inoculants as biofertilizers, biocontrol, or stress protection products. The plant microbiome has not only an impact on plant health, it also influence the human microbiome, e.g. by raw-eaten fruits, vegetables and herbs. Moreover, it can have a positive impact on the microbiome of our built environment. Taken together, plant microbial networks are powerful networks with an impact on ecohealth. Colonization by bacteria of the zone surrounding plant roots (rhizosphere) is crucial to plant productivity, with plants secreting 10-30% of total photosynthate to engineer the rhizosphere to their advantage. In spite of its importance rhizosphere colonization is poorly understood but recent advances in genome sequencing and analysis makes it possible to address this complex topic in exciting new ways. Microarray and metabolic analysis has been used to dissect the composition of the pea root secretome and map the transcriptional response of bacterial to secreted metabolites. Most recently this work has led to identification of the master regulator of attachment of Rhizobium leguminosarum to pea roots.
Views: 245 JXB YouTube
Jason Kralj - Standards for Microbiome and Metagenomics...
 
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Watch this presentation on Labroots at https://www.labroots.com/virtual-event/molecular-diagnostics-2017/agenda In spite of the huge potential impact of microbiome science, current measurement capabilities are insufficient, particularly for translating discoveries and correlations observed in the lab into commercially viable products and services that improve our quality of life. Data are difficult to compare between experimenters, laboratories, or institutions. Emerging capabilities (e.g., next generation sequencing, metabolomics) are new and not fully characterized for microbiome investigations. Reference samples (i.e., for calibration or quality control) that mimic the complexity of naturally occurring communities are not available. Bioinformatic analysis packages and reference databases remain incomplete. At NIST, we are improving microbiome science and supporting the National Microbiome Initiative by developing standards for microbiome measurements that will enable federal, academic, and industry labs to reliably reproduce and advance each other's results. Microbiome standards will support research investigations and commercial translation of microbiome science by providing measurement assurance tools: standardized protocols, reference materials, validated measurements and critically evaluated reference data.
Views: 90 LabRoots
Dr. Emeran Mayer: "The Mind-Gut Connection" | Talks at Google
 
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Dr. Emeran Mayer joins us in the Mountain View Teaching Kitchen to present his new book The Mind-Gut Connection and to talk about how our gut and our brain are inextricably linked; how the microbes living in our gut play a crucial role in this dialogue; and what he recommends to harness this connection. After the talk, Dr. Mayer answers questions from our moderator, Liv Wu, and from the audience. Get the book here: https://goo.gl/lJiO8k Moderated by Liv Wu.
Views: 8492 Talks at Google
Larry Smarr - The Human Microbiome and the Revolution in Digital Health
 
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2014.01.22 The human body is host to 100 trillion microorganisms, ten times the number of cells in the human body and these microbes contain 100 times the number of DNA genes that our human DNA does. The microbial component of our "superorganism" is comprised of hundreds of species with immense biodiversity. Thanks to the National Institutes of Health's Human Microbiome Program researchers have been discovering the states of the human microbiome in health and disease.
Views: 456 Calit2ube
Special microbes make anti-obesity molecule in the gut
 
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Microbes may just be the next diet craze. Researchers have programmed bacteria to generate a molecule that, through normal metabolism, becomes a hunger-suppressing lipid. Mice that drank water laced with the programmed bacteria ate less, had lower body fat and staved off diabetes — even when fed a high-fat diet — offering a potential weight-loss strategy for humans. Subscribe! http://bit.ly/AmerChemSOc Facebook! https://www.facebook.com/AmericanChem... Twitter! https://twitter.com/ACSpressroom You might also like: Press Conferences from #NOLA: https://www.youtube.com/watch?v=SJoMMnl6eKs&list=PLLG7h7fPoH8Jyqtiv9eOb3nfas3LDxG_p Press Conferences from #Philadelphia: https://www.youtube.com/watch?v=5slYZZAFYQs&list=PLLG7h7fPoH8JNXYo-fCwTiFOqba9VEpv9 Press Conferences from #SanDiego: https://www.youtube.com/watch?v=C9Y17zZoqB0&list=PLLG7h7fPoH8L8o4Um_LZTS2lHxorDgHAH Music: From Audioblocks Produced by the American Chemical Society, the world’s largest scientific society. ACS is a global leader in providing access to chemistry-related information and research through its multiple databases, peer-reviewed journals and scientific conferences. Join the American Chemical Society! https://bit.ly/Join_ACS
Mapping the Urban Microbiome, Genome, and Metagenome - AMNH SciCafe
 
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Every year, scientists are learning more and more about the human microbiome, or the collection of microorganisms and bacteria that live in and on our bodies. But what about our “macrobiomes” - the microorganism communities that live in our environments? In this SciCafe, geneticist Chris Mason talks about his desire to get the gene sequence of every thing and place he sees, and the ways in which we can use the information we get from our bodies as well as our environments. This SciCafe took place at the Museum on February 4, 2015. To listen to the full lecture, download the podcast here: http://www.amnh.org/explore/news-blogs/podcasts/scicafe-mapping-the-urban-microbiome-genome-metagenome The SciCafe series is proudly sponsored by Judy and Josh Weston. This project is supported by the Science Education Partnership Award (SEPA) program of the National Institutes of Health (NIH). This video and all media incorporated herein (including text, images, and audio) are the property of the American Museum of Natural History or its licensors, all rights reserved. The Museum has made this video available for your personal, educational use. You may not use this video, or any part of it, for commercial purposes, nor may you reproduce, distribute, publish, prepare derivative works from, or publicly display it without the prior written consent of the Museum. © American Museum of Natural History, New York, NY
Genomics of Microbes and Microbiomes - Julie Segre (2012)
 
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April 25, 2012 - Current Topics in Genome Analysis More: http://www.genome.gov/COURSE2012
Keynote 2: The Modern vs. Ancestral Microbiome - Maria Dominguez-Bello
 
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July 24-26, 2013 - Human Microbiome Science: Vision for the Future More: http://www.genome.gov/27554404
The Human Microbiome Project - Lita Proctor
 
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February 12, 2018 - National Advisory Council for Human Genome Research (Open Session). More: https://www.genome.gov/27570550
Microbiome/Metagenome Analysis Workshop: DADA2
 
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Analysis of 16S data using DADA2 presented by Damien Cabral For more info: https://www.brown.edu/academics/computational-molecular-biology/cbc-microbiomemetagenome-analysis-workshop Tuesday, November 7th 2017 Brown University
Views: 1779 Brown University
Genomics of Microbes and Microbiomes - Julie Segre (2016)
 
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May 18, 2016 - Current Topics in Genome Analysis 2016 More: http://www.genome.gov/CTGA2016
What Is Irritable Bowel Syndrome?
 
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I’m sure you know the feeling, even though you may not know the name. Irritable bowel syndrome or Spastic Colon is one of the most common intestinal disorders. Most doctors and patients find it hard to clearly define just what Irritable Bowel Syndrome is. It used to be a diagnosis of exclusion where the doctor would finally label your symptoms as IBS when they couldn’t think of anything else to call it,But IBS research has advanced so much in the last 5 years that it has cleared the path to a much fuller understanding of the cause and effects of IBS.
Views: 329 Dr. Don Davis
Friends with Benefits: Microbes, Diet & Me - Eric Alm, PhD and Tim Spector, MD
 
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ON BEING HUMAN (series title) Exploring the layers of the human experience LEE AND NILE ALBRIGHT ANNUAL SYMPOSIUM Friends with Benefits: Microbes, Diet & Me Wednesday, October 7, 2015 Eric Alm, PhD, Karl Van Tassell Career Development Associate Professor of Bioengineering at MIT, Associate Member of the Broad Institute, and Co-Director of the Center for Microbiome Informatics and Therapeutics; and Tim Spector, MD, Professor of Genetic Epidemiology at King’s College London, Honorable Consultant Physician at Guy’s and St. Thomas’ Hospital, and author of The Diet Myth: The Real Science Behind What We Eat Why is it that one person can eat a meal and gain weight and another eats exactly the same food and loses pounds? Genes are part of the answer, but the latest science shows another vital aspect that lies within us: microbes. This microbiome of 100 trillion “bugs” co-habit our bodies and influence our biology in myriad ways that scientists are beginning to understand. Tim Spector leads the largest microbiome project in the United Kingdom, using genetic sequencing to study the gut bacteria of 5,000 twins, and founded the British Gut project in 2014. Eric Alm studies microbial ecology, how our microbes can improve human health, and how we can turn our “bugs” into drugs. Learn how your diet affects your microbes and your microbes affect your diet. Funding provided by the Lee and Nile Albright Annual Symposium Fund. Additional funding provided by the Lowell Institute.
Views: 17715 BostonMOS
John Ioannidis: The role of bias in nutritional research
 
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John P.A. Ioannidis, C.F. Rehnborg Professor in Disease Prevention in the School of Medicine, and Professor, by Courtesy, of Statistics and Biomedical Data Science, Stanford University, presented "The role of bias in nutritional research" at the Swiss Re Institute's "Food for thought: The science and politics of nutrition" conference on 14 - 15 June 2018 in Rüschlikon. Find out more about the event: http://institute.swissre.com/events/food_for_thought_bmj.html
Views: 3678 Swiss Re
NCBI Minute: How to Submit Your 16S rRNA Data to NCBI
 
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Presented March 29, 2017. This short webinar guides you through the submission of prokaryotic 16S rRNA sequences to GenBank using one of the new Submission Wizards. You will learn what information you need to get started, and see a demonstration of the submission interface with a real example. You'll also learn about common problems you may encounter, how to deal with them and what to expect after you click the submit button. https://submit.ncbi.nlm.nih.gov/subs/genbank/ To get video updates, subscribe to the NCBI YouTube channel: www.youtube.com/ncbinlm
Views: 8536 NCBI
The NIH Common Fund's Human Microbiome Project
 
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No Longer Germ Warfare. An interview with Dr. Julie Segre, NIH Intramural Researcher, The NIH Common Fund's Human Microbiome Project.
Views: 9753 NIHOD
From Correlation to Causation in Human Microbiome Studies - Rob Knight
 
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July 24-26, 2013 - Human Microbiome Science: Vision for the Future More: http://www.genome.gov/27554404
The Science Behind Artificial Sweeteners | Are They Safe? Are They Making Us Fat?
 
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Get 3 Months of Unlimited Access for Only $0.99 ‣ ▹http://skl.sh/sbuttermore99 EXCLUSIVE offer just for JANUARY! ▹Skillshare is an online learning community for creators with over 17,000 courses videography, business, photography, and more. Please Subscribe! http://bit.ly/substephaniebuttermore This video did take me a long time to create so please let me know if you like this type of content from me! Time Stamps: ▹Acceptable Daily Intake Explanation‣ 2:07 ▹Aspartame ‣ 3:07 ▹Sucralose ‣ 6:33 ▹Acesulfame Potassium ‣ 7:54 ▹Saccharin ‣ 10:08 ▹Stevia ‣ 10:55 ▹Weight Gain/Increased Appetite ‣ 12:47 References: •National Cancer Institute- Artificial Sweeteners and Cancer ▹https://www.cancer.gov/about-cancer/causes-prevention/risk/diet/artificial-sweeteners-fact-sheet •American Cancer Society – Aspartame ▹https://www.cancer.org/cancer/cancer-causes/aspartame.html •Magnuson et al., 2016 – Biological fate of low-calorie sweeteners ▹https://academic.oup.com/nutritionreviews/article-lookup/doi/10.1093/nutrit/nuw032 •Mallikarjun & Sieburth, 2015 – Aspartame and Risk of Cancer: A Meta-analytic Review ▹http://www.tandfonline.com/doi/full/10.1080/19338244.2013.828674?scroll=top&needAccess=true •Sharma et al., 2016 – Artificial sweeteners as a sugar substitute: Are they really safe? ▹https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4899993/ •Ma et al., 2009 – Effect of the artificial sweetener, sucralose, on gastric emptying and incretin hormone release in healthy subjects ▹https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2670679/ •Bian et al., 2017 – The artificial sweetener acesulfame potassium affects the gut microbiome and body weight gain in CD-1 mice ▹http://journals.plos.org/plosone/article/authors?id=10.1371/journal.pone.0178426 •Urban et al., 2013 - Steviol glycoside safety: Is the genotoxicity database sufficient? ▹http://www.sciencedirect.com/science/article/pii/S0278691512007533?via%3Dihub •Tate et al., 2012 – Replacing caloric beverages with water or diet beverages for weight loss in adults ▹https://www.ncbi.nlm.nih.gov/pubmed/22301929 •Anton et al., 2010 – Effects of stevia, aspartame, and sucrose on food intake, satiety, and postprandial glucose and insulin levels ▹https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2900484/ •Fitch & Keim, 2012 – Position of the Academy of Nutrition and Dietetics: Use of Nutritive and Nonnutritive Sweeteners ▹http://jandonline.org/article/S2212-2672(12)00325-5/fulltext •Schiffman et al., 1987 – Aspartame and Susceptibility to Headache ▹http://www.nejm.org/doi/full/10.1056/NEJM198711053171903 ------------------------------- My Last Video▹ ▹2017 | The Year That Changed Everything‣ https://youtu.be/8MWY-GL8zG8 ------------------------------- Recommended Videos▹ ▹How I Got My PhD ‣ https://youtu.be/pGLfcr181l0 ▹Day in The Life of a PhD ‣ https://youtu.be/V8Bs0RH6ytM ------------------------------- FOLLOW ME ▹ INSTAGRAM ‣ http://instagram.com/stephanie_buttermore SNAPCHAT ‣ http://snapchat.com/add/steph_butter FACEBOOK ‣ http://facebook.com/stephaniebuttermore JEFF'S INSTAGRAM ‣ http://instagram.com/jeffnippard JEFF'S CHANNEL ‣ https://www.youtube.com/jeffnippard ------------------------------- CONTACT ME ▹ BUSINESS ONLY EMAIL: [email protected] ------------------------------- FAQs ▹ 1.What is your ethnicity? ‣ Mom is Thai and Dad is Canadian..Eh? 2. How tall are you? ‣ 5'4" 3. How old are you? ‣ 27 3. What are you researching? ‣ Watch my PhD Day in the life video ▹ http://bit.ly/dayasaphd 4. Is Jeff my boyfriend? ‣ Duh 5. Is that your real hair? ‣ Yes ___ AYOOO!! My name is Stephanie Buttermore and in a few words I am a fitness enthusiast but a scientist at heart! Just obtained my Ph.D. in pathology and cell biology with a research focus on the molecular mechanisms that drive ovarian cancer progression. Hope you stick around! xoxo
Views: 148911 Stephanie Buttermore
The cryo-EM revolution
 
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The cryo-EM revolution Air date: Wednesday, April 11, 2018, 3:00:00 PM Category: WALS - Wednesday Afternoon Lectures Runtime: 01:01:57 Description: NIH Director's Wednesday Afternoon Lecture Series The Annual G. Burroughs Mider Lecture Research in the Subramaniam lab over the last decade has been guided by the vision that emerging tools in 3D electron microscopy hold great promise for imaging cells, viruses and protein complexes at high resolution in their native states, thus bridging a major gap in structural biology. In his talk, he will review examples of recent progress ranging from determination of protein structures at atomic resolution to imaging viruses, cells and tissue at nanometer resolution. For more information go to https://oir.nih.gov/wals/2017-2018/ Author: Sriram Subramaniam, Ph.D., Senior Investigator, Laboratory of Cell Biology, National Cancer Institute, NIH Permanent link: https://videocast.nih.gov/launch.asp?23812
Views: 546 nihvcast
Does Candida Cause Acne Rosacea
 
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Discover if there is a link between candida and acne rosacea. Can candida cause severe acne rosacea? Greetings. New Zealand naturopath, Eric Bakker. Author of Candida Crusher and formulator of the Canxida range of supplements. Thanks for checking out my video. Question here by Pasqual from Quebec, Canada. Pasqual's asking me, "Eric, does Candida cause acne rosacea?" Pasqual, there is definitely a link between Candida and acne rosacea. That's been proven now. I've looked at many different kinds of studies from PubMed, InBase, and CINAHL, and I've had a really good look through different databases online after I got your email. Also, in Singapore, there is a skin clinic there where they've found a very clear-cut link between the gut microbiota to cleaning up the gut with patients and the skin. I've noticed this in my clinic now for probably over 20 years. I've treated over a thousand cases of psoriasis successfully, but I also treat a lot of acne rosacea and many other different kinds of skin conditions. There is a huge link between the gut and the skin. A very interesting case I recently had was a male patient from Portugal, a 38-year-old male with seriously bad acne rosacea. Basically, I didn't even look at the skin. I just treated the gut and we had an outstanding result. We found that this guy had incredible amounts of Candida albicans, Candida glabrata, and also Blastocystis hominis. And once we cleared up these things and we found that with pre and post stool testing, we had an incredibly high success rate with eradication, the skin entirely cleared up. He's a very lovely man and now he excitedly Skyped me recently. He said, "I've now got a girlfriend. I've got a Swedish girlfriend. I'm so excited." I don't know if you've seen how bad acne rosacea can get. When you've got bad acne rosacea, you've got no self-confidence. For this guy after 10 or 15 years to get his skin finally cleared up, his confidence was like in overdrive and he was so excited to tell me all about his girlfriend. How did this association occur? Well, in Singapore at the Dermoclinic what they found out was that Candida could actually produce different kinds of chemicals that upregulate or stimulate the production of inflammatory cytokines. So what that means in normal English is Candida can stir the hell out of the immune system and the immune system, in turn, then can create chemicals that can create dilation of the skin, the blood vessels, flushing, redness, irritation, infections, all kinds of things can occur. There is certainly a link there. If anyone is watching this who has got acne rosacea, my strong advice for you is (A) improve the upper digestion because many people with acne rosacea have got hypochlorhydria or underactive stomach, (B) ensure that the pancreas is working properly and it will work better when the stomach is working better, (C) make sure that you've cleared up food allergies or food intolerances. Sometimes it's worthwhile doing an ELISA test, so an IgG, IgE food allergy test. The best one, I think, is from US Biotech in Seattle. It's s serum test, (D) if you've got a really bad problem like my patient in Portugal, get a proper stool test done, a comprehensive one to assess what kind of bugs you've got in the gut. So then, we can go about cleaning up the gut and (E) kick back and enjoy life. Because if you do all of those things properly, there is a big chance we can clear up the acne rosacea profoundly and you can lead a relatively normal life with a good amount of self-esteem. I hope that answers your question, Pasqual. In my opinion, there is a massive link between acne rosacea and a yeast infection. Don't forget, while I've got your attention, to please subscribe to my channel. Check out my 1,000+ articles at yeastinfection.org and make sure you do my quiz. I've got the world's best online quiz. I've also got a very nice supplement for people with acne rosacea. It's called Canxida Restore. It's a great probiotic enzyme formula. Be sure to take my antifungal Canxida Remove and Canxida Restore. These two products are going to help you hugely with acne rosacea, especially if you've done some stool testing and been checked out and checked positive to have bugs. Candida Diet: https://www.youtube.com/watch?v=R9327DEOWcc Candida Cleanse: https://www.youtube.com/watch?v=VwUBdS-dPZM Candida Foods To Avoid: https://www.youtube.com/watch?v=8_9ntEF2_YU Candida Foods To Eat: https://www.youtube.com/watch?v=Ccm4X8wKRPI
Views: 4674 Candida Crusher
The Human Microbiome: Emerging Themes at the Horizon of the 21st Century (Day 3)
 
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The Human Microbiome: Emerging Themes at the Horizon of the 21st Century (Day 3) Air date: Friday, August 18, 2017, 8:15:00 AM Category: Conferences Runtime: 04:59:15 Description: The 2017 NIH-wide microbiome workshop will strive to cover advances that reveal the specific ways in which the microbiota influences the physiology of the host, both in a healthy and in a diseased state and how the microbiota may be manipulated, either at the community, population, organismal or molecular level, to maintain and/or improve the health of the host. The goal will be to seek input from a trans-disciplinary group of scientists to identify 1) knowledge gaps, 2) technical hurdles, 3) new approaches and 4) research opportunities that will inform the development of novel prevention and treatment strategies based on host/microbiome interactions over the next ten years. Author: NIH Permanent link: https://videocast.nih.gov/launch.asp?23424
Views: 1761 nihvcast
Metaproteomics For The Future: ASMS 2017 presentation on metaproteomics
 
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Metaproteomics For The Future: Democratizing functional analysis of microbiomes via community-based informatics development and dissemination. The impact of microbiomes (genes expressed by microbiota) on human health and environment is getting increasingly appreciated. Microbiome research is dominated by taxonomic-centered estimations using metagenomic 16S rRNA-based approaches. Functional characterization with metaproteomics analysis (study of expressed microbial proteins) stands to more accurately and mechanistically describe microbial responses in these dynamic systems. However, metaproteomic informatics remains an emerging research area, which requires numerous software tools to manage, analyze and interpret data. Consequently, this growing field will need deliberate, international coordination to create the kinds of robust software that will facilitate metaproteomic informatics. Here, we propose a community-based and innovative approach that we believe will result in robust analytical workflows and will help in advancing and broadening the reach of metaproteomics. A collaborative informatics initiative to develop a suite of validated software and robust Galaxy workflows for advanced metaproteomics informatic analysis.
Views: 165 Pratik Jagtap
Barriers to open sharing of research data using social tools?  Q and A with Dr. Pieter Dorrestein
 
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In this clip, Dr. Pieter Dorrestein discusses the challenge of encouraging researchers to share data openly using collaborative tools. Dr. Dorrestein is professor at the Skaggs School of Pharmacy and Pharmaceutical Sciences at the University of California at San Diego. He delivered a lecture at NIH in April 2015 on “Social Networks For Molecular Analysis.” Watch his lecture here: http://videocast.nih.gov/Summary.asp?... Q: Have there been barriers to researchers sharing their data openly? A: So yes, I think the scientific community has been trained to be quite afraid of sharing data beforehand. There’s a couple of ways that you can limit that concern. One is if you upload raw data you can still make cross-correlations with other data sets. There’s limited context with what’s put in and so. But our idea is with our tool is to actually make the analysis so powerful that if you don’t make your data publicly accessible, you can spend many more man-years to figure out the same thing. And so by making it publicly accessible, you actually get way more information than we would otherwise be able to get. And so that’s a real incentive for a lot of people to make the data publicly accessible. And I think it’s well, should it be mandated? Probably not. It really should be driven from the standpoint that these tools are so useful that they will want to use it. Now if you look at the breakdown, however, of people who are using it, you can clearly see that this younger generation is not afraid to share. They don’t have this fear of being scooped. They know how to work collaboratively, and that to me is really an exciting direction that the scientific community is going to. We’re no longer isolated silos. No, we work as a group even though each individual person can put out their own publication, we can still cross-learn from all the information that was provided.
Views: 128 NCCIH
20180409 4 OTUs from 16S rRNA
 
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Slides for this lecture can be found at https://drive.google.com/open?id=1aAXuC7__6C00Xprt-YjMS7lbGV8zGYaE This lecture on amplicon sequencing informatics was the second of three to describe the bioinformatics of Next-Generation Sequencing (NGS) for the B.Sc. Honours program in Biotechnology at the University of the Western Cape. Microbiome research frequently relies upon an assessment of alpha and beta diversity of species, as approximated by Operational Taxonomic Units (OTUs). OTUs, in turn, represent sets of 16S rRNA sequences that are 97% similar to each other, clustered from amplicon sequencing by software such as MOTHUR, QIIME, and UPARSE. These clusters can then be matched to 16S rRNA sequences that have been associated with taxonomic information, sometimes to the genus or species level. This association comes from the use of a 16S database such as RDP, SILVA, or Greengenes. Rarefaction curves can then evaluate the extent to which sequencing represents a comprehensive catalog of species for the sample.
Views: 139 David Tabb
Stephen Porges: The Polyvagal Theory & The Vagal Nerve – #264
 
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Stephen Porges comes to Bulletproof Radio to share his pioneering research on the vagus nerve, how the cues it receives play a major role in stress, social behavior and the nervous system and some tips on how to improve its response. Dr. Porges is a Distinguished University Scientist at Indiana University where he directs the Trauma Research Center in the Kinsey Institute. He is Professor of Psychiatry at the University of North Carolina, Professor Emeritus at the University of Illinois at Chicago, where he directed the Brain-Body Center in the Department of Psychiatry, and Professor Emeritus at the University of Maryland, where he chaired the Department of Human Development and directed the Institute for Child Study. In 1994 he proposed the Polyvagal Theory, a theory that links the evolution of the mammalian autonomic nervous system to social behavior and emphasizes the importance of physiological state in the expression of behavioral problems and psychiatric disorders. Enjoy the show! Featured Stephenporges.com - http://stephenporges.com/ A selection of Dr. Porges publications - http://stephenporges.com/index.php/scientific-articles Talks by Dr. Porges on Youtube - https://www.youtube.com/results?search_query=stephen+porges Resources Polyvagal Theory - https://en.wikipedia.org/wiki/Polyvagal_Theory Autonomic nervous system - http://www.merckmanuals.com/home/brain-spinal-cord-and-nerve-disorders/autonomic-nervous-system-disorders/overview-of-the-autonomic-nervous-system Neuroception - http://eric.ed.gov/?id=EJ938225 Hypoxia - http://www.webmd.com/asthma/guide/hypoxia-hypoxemia Phylogenetics - https://en.wikipedia.org/wiki/Phylogenetics Vagus nerve - http://emedicine.medscape.com/article/1875813-overview Heart rate variability - http://circ.ahajournals.org/content/93/5/1043.full Vagal tone - http://www.encyclopedia.com/doc/1O6-vagaltone.html Apnea - http://kidshealth.org/parent/medical/lungs/aop.html Bradycardia - http://www.mayoclinic.org/diseases-conditions/bradycardia/basics/definition/con-20028373 Homeostatic functions - http://www.wisegeek.com/what-are-the-different-types-of-homeostatic-functions.htm Nucleus ambiguus - http://www.britannica.com/science/nucleus-ambiguus Myelination - http://tweenparenting.about.com/od/physicalemotionalgrowth/a/Definition-of-Myelination.htm Myelinated vagus nerve - http://masgutovamethod.com/content/overlays/stephen-porges.html Pranayama yoga - http://www.yogajournal.com/category/poses/types/pranayama/ Cranial nerve 5 - https://12cranialnerves.wordpress.com/cranial-nerve-5-trigeminal-nerve/ Cranial nerve 7 - https://12cranialnerves.wordpress.com/cranial-nerve-7-facial-nerve/ Dr. Sue Carter - http://www.kinseyinstitute.org/about/carter.html Dr. Lana - https://www.betterbabybook.com/better-baby-coaching/ Health registry database in Nordic countries - http://www.norden.org/en/fakta-om-norden-1/figures-and-statistics HIV’s impact on vagal recognition - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742727/ Ventral vagal state - http://reichandlowentherapy.org/Content/Vegetative/ventral_shift.html The Center for Compassion and Altruism Research and Education On Combat - https://www.youtube.com/user/CcareStanford Neuroception - http://www.amazon.com/On-Combat-Psychology-Physiology-Conflict-ebook/dp/B008068P8K Bulletproof Heart rate variability training - https://www.bulletproofexec.com/how-to-biohack-your-courage-using-the-wizard-of-oz-and-heart-rate-variability/ HeartMath Institute - https://www.bulletproofexec.com/how-heartmath-training-helps-you-live-longer/ Bulletproof Road Map - https://www.bulletproofexec.com/the-complete-illustrated-one-page-bulletproof-diet/ Bulletproof Cookbook - http://www.amazon.com/Bulletproof-Diet-Cookbook-bulletproof-recipes-ebook/dp/B00QECPD9Q?utm_source=post&utm_medium=blog&utm_campaign=pod264
Views: 62715 Bulletproof
Import metagenomics data (BioNumerics 7)
 
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This video shows how to import metagenomics data in a BioNumerics 7 database.
Views: 197 BioNumerics
What biodiversity means for beer (and medicine) | Even Skjervold | TEDxGreenville
 
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This Upstate bioengineer demonstrates how nature can help us make great beer - and solve some of the world's major challenges. Born and raised in Norway, Even came to the US seeking an environment that fostered creation and progress. He wanted to find a cure for cancer through nanotechnology. After received his undergraduate degree in bioengineering from Clemson University, he decided to expand his horizons with a business degree that would allow him to increase his impact. By day, Even works as a “big picture” engineer for companies that make medical devices, by night —an entrepreneur. His first venture is SouthYeast Labs, where he and a former teacher produce custom local yeast strains for craft breweries. The process of discovering so many diverse microbes through SouthYeast inspired this talk and prompted Even to ask: What else is out there, just waiting to be found? This talk was given at a TEDx event using the TED conference format but independently organized by a local community. Learn more at http://ted.com/tedx
Views: 498 TEDx Talks
Seeing is understanding: The EMBL Imaging Technology Centre
 
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This video gives a first impression of the ITC building at EMBL and shows the potential in the new imaging technologies. The new centre for light and electron microscopy will be a unique service facility for the life sciences and unite cutting-edge equipment, experts and data analysis.
Dr. Eric Verdin on Ketogenic Diet Longevity, Beta-Hydroxybutyrate, HDAC Inhibitors & NAD+
 
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Eric M. Verdin, M.D. is the fifth president and chief executive officer of the Buck Institute for Research on Aging and is a professor of Medicine at UCSF. Dr. Verdin's laboratory focuses on the role of epigenetic regulators in the aging process, the role of metabolism and diet in aging and on the chronic diseases of aging, including Alzheimer’s, proteins that play a central role in linking caloric restriction to increased healthspan, and more recently a topic near and dear to many of you, ketogenesis. He’s held faculty positions at the University of Brussels, the NIH and the Picower Institute for Medical Research. ▶︎ Get the show notes! https://www.foundmyfitness.com/episodes/eric-verdin ▶︎ Dr. Verdin's Twitter http://twitter.com/ericverdin ▶︎ Verdin Lab - Buck Institute for Research on Aging http://verdin.buckinstitute.org/ --- Links related to FoundMyFitness: ▶︎ Subscribe on YouTube: http://youtube.com/user/FoundMyFitness?sub_confirmation=1 ▶︎ Join my weekly email newsletter: http://www.foundmyfitness.com/?sendme=lifestyle-heuristic ▶︎ FoundMyFitness Genetics: http://www.foundmyfitness.com/genetics ▶︎ Crowdfund more videos: http://www.foundmyfitness.com/crowdsponsor ▶︎ Subscribe to the podcast: http://itunes.apple.com/us/podcast/foundmyfitness/id818198322 ▶︎ Twitter: http://twitter.com/foundmyfitness ▶︎ Facebook: http://www.facebook.com/foundmyfitness ▶︎ Instagram: http://www.instagram.com/foundmyfitness
Views: 81808 FoundMyFitness
Making friends with your microbes: Kevin Spyker at TEDxJerseyCity
 
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In the spirit of ideas worth spreading, TEDx is a program of local, self-organized events that bring people together to share a TED-like experience. At a TEDx event, TEDTalks video and live speakers combine to spark deep discussion and connection in a small group. These local, self-organized events are branded TEDx, where x = independently organized TED event. The TED Conference provides general guidance for the TEDx program, but individual TEDx events are self-organized.* (*Subject to certain rules and regulations)
Views: 1439 TEDx Talks
Dr. Barnes - Healing Leaky Gut Protocol - 14 Minutes
 
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Here is the Protocol: Rebuild gut: (The 4 Rs) All four steps are done concurrently, they are just separated so you know what each part is specifically doing. *1. Remove (90 Days) - - The 5 causes: Food sensitivities (Follow testing results - Cyrex Array 10 - IGG,IGA) - Email [email protected] for testing info - Sugar and ALL Grains - Processed foods (bad fats, caffeine, alcohol, gluten, soy) - Dairy – even organic and Raw - Avoid Stress! If present Address Parasites - do stool sample through Doctor’s Data - Remove the triggers - (NSAIDS) pills poke holes in gut – Tylenol, aspirin, ibuprofen - Avoid antibiotics at all costs! http://www.theatlantic.com/health/archive/2015/11/taking-antibiotics-can-change-the-gut-microbiome-for-up-to-a-year/415875/ *2. Repair- Begin taking: Vitamin D3 daily - If below 60 ng/mol - 10,000 ius daily for 30-60 days then retest 5,000 IUs daily for maintenance Taken in the morning, with a meal (fat) Designs For Health (DFH) Aloe Vera 200x - 3 x day before meals + aloe vera gel - 2x/day DFH L-glutamine daily 3gm - breakfast (smoothie or just water) Max GI - 2 capsules on empty stomach - Best in AM or after eating something you shouldn't have :). This helps with Small intestinal bacterial overgrowth and bloating/gas issues DFH Gastro Mend – HP - 2 Capsules in AM and 2 in PM 3. Restore- Begin taking: - Probiotics -150 billion a day minimum, Max GI, sauerkraut - Proper Nerve Supply- corrective chiropractic adjustments - Fermentable Fibers – (Sweet potato, yam, plantains, yucca, jewish artichokes) - Grassfed, organic butter (butyrate) daily - Increase organic, healthy animal protein - Bullet Proof Coffee - Take DFH PrePhage and Probiophage as directed 4. Replace - - DFH Betaine HCL w pepsin- take 2 capsules per meal - DFH Zinc supreme - take 3 capsules per day (PM) - Max GI – contains digestive enzymes to aid in breakdown All Supplements can be found online https://greenwayhealth.ehealthpro.com/products Max GI can be found here: https://store.maximizedliving.com/scripts/prodView.asp?idproduct=566 For more information visit: http://www.drgregbarnes.com
Views: 34244 Greenway Chiropractic
Pathways To Cures:The Microbiome
 
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What is the Microbiome? Approximately 90% of the cells that are on and inside your body aren't you. Those cells are the trillions of microscopic bacteria and viruses that make up the human microbiome. The gut's microbiome contains a mix of enormously complex bacteria, viruses and fungi -- but only a limited number of these microbial agents have been identified and their functions are still largely unknown. Learn why the Microbiome is important to cures at: www.ccfa.org.
clinical treatment - Diseasses Treatment
 
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Introduction: "Clinical Treatment" Provide you one of the best education about Treatment of Diseases and remained the first choice of Doctors especially House Officers, Medical Officers and Training Medical Officers. Features: - Treatment Of Diseases - Quick Search of Disease - Aims Of Treatment -General Measures - Pharmacological Treatment - Criteria for Referral - Doses of Drugs - Route Of administration of Drugs - Various Brand Names of Drugs used in Treatment of Diseases Contents: It covers the Treatment of Diseases Of Following systems: -Cardiovascular system -Respiratory System -Dermatology -Gynaecology and Obstetrics -Dentistry -Urogenital System -Nervous System -Hepatobiliary System -Poisoning -Endocrine System -Hematology -GastroIntestinal System -Infectious Diseases -Musculoskeletal System -Psychitary -Sexually Transmitted Diseases
Views: 5328 Fari Marwat
Making Universal Donor Blood From Other Blood Types | Headline Science
 
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Stephen Withers, from the University of British Columbia, reports on enzymes that remove A or B antigens from red blood cells 30 times more efficiently than previously reported enzymes. This could help scientists make universal donor blood from other blood types. These findings were presented at the 256th American Chemical Society National Meeting in Boston, MA. Video produced by Research Square https://www.researchsquare.com/ Subscribe! http://bit.ly/AmerChemSOc Facebook! https://www.facebook.com/AmericanChem... Twitter! https://twitter.com/ACSpressroom Music by Kevin MacLeod Produced by the American Chemical Society, the world’s largest scientific society. ACS is a global leader in providing access to chemistry-related information and research through its multiple databases, peer-reviewed journals and scientific conferences. Join the American Chemical Society! https://bit.ly/Join_ACS
Multi-omics of the Human microbiome - Filling in the Missing Links - Janet Jansson
 
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July 24-26, 2013 - Human Microbiome Science: Vision for the Future More: http://www.genome.gov/27554404

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