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ELISA Tutorial 5: Preparing ELISA Data in Excel for Analysis with GraphPad Prism
 
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Here, we demonstrate the first steps of data analysis for some typical ELISA data. The procedure shown is as follows: 1) Compare the arrangement of our samples on the 96-well plate with the absorbance measurements obtained from the plate reader. 2) Subtract out the blank measurements from all the entire plate. 3) Arrange the absorbance measurements of the standards next to their corresponding known concentrations. 4) Place the absorbance measurements of the samples below the absorbance measurements of the standards. In the next video of this series, we will demonstrate how to interpolate the concentration of our samples using software called GraphPad Prism. Click here for a free 30-day trial of GraphPad Prism - http://protocol-place.com/Prism This video and other protocols can be found at our website, the "Protocol Place" - http://protocol-place.com/ A full ELISA protocol can be found at http://protocol-place.com/assays/sandwich-elisa-protocol/ This video is a part of our ELISA Tutorial Series. Here are all of the videos in this series: ELISA Tutorial 1: Understand How an ELISA Works - http://youtu.be/nNjlBCnpGZ4 ELISA Tutorial 2: Coating and Blocking the ELISA Plate - http://youtu.be/AmG7FBolfdc ELISA Tutorial 3: Preparing and Adding Samples to the ELISA Plate - http://youtu.be/darrx6F0wsg ELISA Tutorial 4: Finishing the Assay (Sandwich ELISA) - http://youtu.be/zI4khIJhCd8 ELISA Tutorial 5: Preparing ELISA Data in Excel for Analysis with GraphPad Prism - http://youtu.be/l9tO81ZCeRg ELISA Tutorial 6: How to Analyze ELISA Data with GraphPad Prism - http://youtu.be/5IqqpKSnXfI Competitive ELISA Tutorial 1: How a Competitive ELISA Works - http://youtu.be/Kb26nQVMHds Competitive ELISA Tutorial 2: How to Use Calbiotech's Competitive ELISA Kits - http://youtu.be/3E_U_4Z2dUc Competitive ELISA Tutorial 3: Analyzing Typical ELISA Data in Excel - http://youtu.be/s2t0jiWxiDI We hope you enjoy watching and benefit from our tutorials. If so, please take a minute to "like," or better yet, share them with others! Thanks for watching! Youssef Farhat Protocol Place: http://protocol-place.com ***Check out some of our other tutorials via the links below*** Competitive ELISA Tutorials - - - http://www.youtube.com/watch?v=Kb26nQVMHds&list=PLR4wfoQ4HbymisBtft-i-QTsyRDMFymC3 ELISA Tutorials - - - http://www.youtube.com/watch?v=nNjlBCnpGZ4&list=PLR4wfoQ4HbynbS01zeuBV-awsOAxDPhYO Gelatin Zymography Tutorials - - - http://www.youtube.com/watch?v=MF2sWQSaBWg&list=PLR4wfoQ4Hbykrj7rxk6i5jzkjtvTtZUVx
Views: 45635 protocolplace
Antibody test (quantitative tests)
 
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For more information, log on to- http://shomusbiology.weebly.com/ Download the study materials here- http://shomusbiology.weebly.com/bio-materials.html Source of all articles published in description is Wikipedia. Thanks to original content developers. Link- http://en.wikipedia.org/wiki/Main_Page This video tutorial talks about different immunological assays and antibody test methods and their importance in clinical biology. It also explains different features of antigen antibody reactions and their role in identifying diseases.
Views: 13539 Shomu's Biology
TCID50 Preparation
 
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This video was prepared by the Teaching Support team for The University of Western Australia's School of Pathology and Laboratory Medicine (PaLM). Credits: Actors: Melissa Andrade Voice-over: Sarah Power
Views: 8466 Biomedical Science
Microplate Reader Data and Elisa Data Analysis Does Not Get Any Easier
 
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The goal of creating this video demo is to demonstrate how quick and easy it is to do curve-fitting quantitative analysis on microplate reader data or elisa data with MasterPlex ReaderFit by Hitachi Software MiraiBio Group. The video is in real-time without any fast-forwarding or fancy editing so users can get a sense of the real experience. The input file used is a simple Excel table with my raw RLU values. Fasten your seatbelts. Were going to get from raw data to calculated concentrations in less than 2 minutes!
Views: 15679 aliumiraibio
Assay of viral infectivity
 
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'Assay of viral infectivity' is video 2 from week 2 of my 2013 Coursera course 'How viruses work'.
Views: 8229 Vincent Racaniello
ELISA Tutorial 6: How to Analyze ELISA Data with GraphPad Prism
 
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Here, we demonstrate how to analyze some typical ELISA using software called GraphPad Prism. This software allows us to effortlessly fit a curve to our standards, and then use that curve to interpolate the concentration of our samples. If you do not already have GraphPad Prism, you can download a free 30-day trial here: http://protocol-place.com/Prism This video and other protocols can be found at our website, the "Protocol Place" - http://protocol-place.com/ A full ELISA protocol can be found at http://protocol-place.com/assays/sandwich-elisa-protocol/ This video is a part of our ELISA Tutorial Series. Here are all of the videos in this series: ELISA Tutorial 1: Understand How an ELISA Works - http://youtu.be/nNjlBCnpGZ4 ELISA Tutorial 2: Coating and Blocking the ELISA Plate - http://youtu.be/AmG7FBolfdc ELISA Tutorial 3: Preparing and Adding Samples to the ELISA Plate - http://youtu.be/darrx6F0wsg ELISA Tutorial 4: Finishing the Assay (Sandwich ELISA) - http://youtu.be/zI4khIJhCd8 ELISA Tutorial 5: Preparing ELISA Data in Excel for Analysis with GraphPad Prism - http://youtu.be/l9tO81ZCeRg ELISA Tutorial 6: How to Analyze ELISA Data with GraphPad Prism - http://youtu.be/5IqqpKSnXfI Competitive ELISA Tutorial 1: How a Competitive ELISA Works - http://youtu.be/Kb26nQVMHds Competitive ELISA Tutorial 2: How to Use Calbiotech's Competitive ELISA Kits - http://youtu.be/3E_U_4Z2dUc Competitive ELISA Tutorial 3: Analyzing Typical ELISA Data in Excel - http://youtu.be/s2t0jiWxiDI We hope you enjoy watching and benefit from our tutorials. If so, please take a minute to "like," or better yet, share them with others! Thanks for watching! Youssef Farhat Protocol Place: http://protocol-place.com ***Check out some of our other tutorials via the links below*** Competitive ELISA Tutorials - - - http://www.youtube.com/watch?v=Kb26nQVMHds&list=PLR4wfoQ4HbymisBtft-i-QTsyRDMFymC3 ELISA Tutorials - - - http://www.youtube.com/watch?v=nNjlBCnpGZ4&list=PLR4wfoQ4HbynbS01zeuBV-awsOAxDPhYO Gelatin Zymography Tutorials - - - http://www.youtube.com/watch?v=MF2sWQSaBWg&list=PLR4wfoQ4Hbykrj7rxk6i5jzkjtvTtZUVx
Views: 54226 protocolplace
Hemagglutination assay
 
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For more information, log on to- http://shomusbiology.weebly.com/ Download the study materials here- http://shomusbiology.weebly.com/bio-materials.html Source of all articles published in description is Wikipedia. Thanks to original content developers. Link- http://en.wikipedia.org/wiki/Main_Page The hemagglutination assay (or haemagglutination assay; HA) is a method of quantification for viruses or bacteria by hemagglutination. It is an easy, simple and rapid method which can be applied to large numbers of samples. The hemagglutination assay and its extension, the hemagglutination inhibition assay, were invented in 1941--42 by American virologist George Hirst.[1][2] The hemagglutination assay of a virus, in contrast to other forms of virus quantification such as a plaque assay or 50% Tissue Culture Infective Dose, does not give any measure of viral infectivity, because no virus replication is required in this assay. The same may not be true when using HA for bacteria. The detailed conditions depend on the type of virus or bacteria being assayed since certain pH values and ionic strengths can impact the activity of the proteins of interest in a difficult to predict manner. Normally, a virus dilution (e.g. 2-fold from 1:4 to 1:4096) will be applied to an RBC dilution (e.g. 0.1% to 0.7% in steps of 0.2%) for approx. 30 min, often at 4 °C, otherwise viruses with neuraminidase activity will detach the virus from the RBCs. Then the lattice forming parts will be counted and the titer calculated. Virus concentration in virions per millilitre = 107 x HA titer.[3] The titer of a hemagglutination assay is determined by the last viable "lattice" structure found. This is because it is at the point where, if diluted anymore, the amount of Virus particles will be less than that of the RBCs and thus not be able to agglutinate them together. For bacteria, depending on species, a bacterial dilution will be applied to an equal part RBC dilution and then incubated for 30 min to an hour at an optimal growth temperature before being observed.[4]
Views: 53571 Shomu's Biology
ELISA
 
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ELISA Readersare laboratory instruments designed to detect biological, chemical or physical events of samples in micro-titer plates. It is a specialized spectrophotometer that is used to determine the absorbance of a large number of samples within a short time. The two types of optics used in ELISA Reader are filter based and mono-chromator based optics. The optical system uses optic fibres to supply light to the micro-plate wells containing the samples.The light beam, passing through the sample has a diameter ranging between 1 to 3 mm.Detection system detects the light coming from the sample.Amplifiers are used to amplify the signal and determine the sample's absorbance. Reading system converts it into data allowing the test result interpretation. It isused for reading the results of ELISA tests, has direct application in immunology and serology. It also confirms the presence of antibodies or antigens of an infectious agent in an organism and detects antibodies from a vaccine or auto-antibodies. Here we have used it for the assay of free phosphorus in maize sample.
Views: 1010 Genomics Lab
Virus Watch: Counting Viruses
 
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In this episode of Virus Watch, I show how to do my favorite assay in all of virology - the plaque assay.
Views: 13382 Vincent Racaniello
ELISA Microplate Reader BIOBASE EL 10A Operation Video
 
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More info, pls contact John at [email protected]
Views: 265 John Xie
Serial Dilution by Column on a 96-Well Microplate
 
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Apricot Designs 96-channel pipettor configured with a 8-channel head for automatic column-by-column serial dilution.
Views: 18955 ApricotDesigns
ELISA data calculation - Excel
 
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Not a complete calculation
Views: 864 jontrip247
ELISA Analysis Plate Layout Software Demostration
 
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http://www.elisaanalysis.com provides free ELISA software for ELISA data analysis. This video is a demonstration of how easy it is to create a plate layout for your ELISA kit using the elisaanalysis.com ELISA software.
Views: 3183 ElisaAnalysis
Hemo-Type+ Analyzer for blood group typing in microplate
 
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High throughput walkaway analyzer for blood group typing with the classical method in microtiter plates. GSG RobotiX, is a company leader in the development, production and distribution of laboratory instruments and solutions for diagnostics and research. GSG RobotiX, a new experience in lab automation. For more information about our robotic solutions, please visit http://www.gsgrobotix.com/
Views: 2534 Lab Automation
Microtiter plate
 
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A microtiter plate or microplate or microwell plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay, the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or even 1536 sample wells arranged in a 2:3 rectangular matrix. Some microplates have even been manufactured with 3456 or even 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape. This video is targeted to blind users. Attribution: Article text available under CC-BY-SA Creative Commons image source in video
Views: 930 Audiopedia
Introduction cultivation of microorganisms and animal cells in microplates (by Wouter Duetz)
 
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Cultivation in microtiter plates (microplates) of bacteria (E. coli, Bacillus, etc.), streptomycetes, fungi and animal cells (e.g. CHO cells), using the technology platform (sandwich covers, clamp systems, orbital shakers) as originally developped by Wouter Duetz and his research group at the Institute of Biotechnology of the ETH Zurich from 1997-2002.
Views: 6999 wouterduetz
Microtiter plate
 
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Fully automated packaging line for pharmaceutical microtiter plates with inline inspection systems and connected to injection moulding machine
Views: 1525 GDO B.V.
Spotlight on Testing: Phospholipase A2 Receptor (PLA2R) IgG Antibody
 
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Our ARUP Institute for Learning Spotlight Series is designed to provide brief educational overviews highlighting test utilization issues, new tests, and testing technology. In this video, Anne Tebo, PhD discusses the Importance of Phospholipase A2 Receptor (PLA2R) IgG Antibody: A Marker for Primary Membranous Nephropathy.
Views: 516 ARUP Laboratories
Vaccines for Lyme Disease - Past, Present, and Future
 
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A Lyme disease vaccine is no longer available. The vaccine manufacturer discontinued production in 2002, citing insufficient consumer demand. This webinar contains presentations that describe the need for a new Lyme disease vaccine; the types of vaccines that are under investigation; and why the previous Lyme disease vaccine failed. Comments on this video are allowed in accordance with our comment policy: http://www.cdc.gov/SocialMedia/Tools/CommentPolicy.html This video can also be viewed at http://www.cdc.gov/lyme/resources/PW4086633.wmv
June 2017 ACIP Meeting - Hepatitis Vaccines
 
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Hepatitis A vaccine background, Cost-effectiveness analysis of catch-up hepatitis A vaccination among unvaccinated/partially-vaccinated children, Updated ACIP routine recommendations for use of hepatitis A vaccine, Updated ACIP recommendations for use of hepatitis A vaccine and immune globulin for post-exposure prophylaxis and for international travelers Comments on this video are allowed in accordance with our comment policy: http://www.cdc.gov/SocialMedia/Tools/CommentPolicy.html This video can also be viewed at https://www.cdc.gov/vaccines/videos/low-res/ACIPJune2017/Hepatitis_Vaccines_LowRes.mp4
Tick-Borne Disease Working Group Meeting - May 10, 2018
 
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Part 1 (Welcome, Introductions, Overview) - https://bit.ly/2ydmepe Part 2 (Disease Vectors, Surveillance, and Prevention Subcommittee Report) - https://bit.ly/2Nq6UeC Part 3 (Pathogenesis, Transmission, and Treatment Subcommittee Report) - https://bit.ly/2NqoNdo Part 4 (Testing and Diagnostics Subcommittee Report) - https://bit.ly/2NpTL57 Part 5 (Access to Care and Support to Patients Subcommittee Report) - https://bit.ly/2P9BsCV Part 6 (Vaccines and Therapeutics Subcommittee Report) - https://bit.ly/2O6v5nu Part 7 (Other Tick-Borne Diseases and Co-Infections Subcommittee Report) - https://bit.ly/2CuhQqD Part 8 (Public Comments and Next Steps) - https://bit.ly/2OGKLgM -- U.S. Department of Health and Human Services (HHS) http://www.hhs.gov HHS Privacy Policy http://www.hhs.gov/Privacy.html
TRACO 2017: Lymphoma and Small molecules
 
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TRACO 2017: Lymphoma and Small molecules Air date: Tuesday, October 17, 2017, 4:00:00 PM Category: TRACO Runtime: 01:48:16 Description: Translational Research in Clinical Oncology (TRACO) Recent advances in understanding cancer biology are beginning to be translated into improvements in diagnosis and treatment of cancer. In the post-genome era, we increasingly rely on strong collaboration between basic and clinical scientists to develop novel approaches for treatment of human disease. The NCI Center for Cancer Research (CCR) is one of the largest cancer research organizations in the world, with more than 200 principal investigators, and has played a major role in developing and implementing many new technologies, such as nanotechnology, next generation sequencing, genomics and proteomics. For more information go to http://ccr.cancer.gov/training/trainee-resources/courses-workshops/traco Author: John T. Schiller, Ph.D., CCR, NCI, NIH and Anton Simeonov, Ph.D., NCATS, NIH Permanent link: https://videocast.nih.gov/launch.asp?23523
Views: 344 nihvcast

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